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NanoLive Inc steve software
Steve Software, supplied by NanoLive Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steve+software/steve+software/pmc12266386-114-12-14
Average 90 stars, based on 1 article reviews
steve software - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Refractive Index:

Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
Article Snippet: .. Refractive index (RI) values were analyzed using STEVE software from Nanolive to digitally stain and distinguish nanoplastics from the cytoplasm inside the cells. .. Immunofluorescence staining was performed as previously described.5,24 For the detection of microglia and M1-activated immune cells, Iba1 (1 : 1000, FUJIFILM Wako Chemical, Osaka, Japan) and CD68 (1 : 100, Bio-Rad, Hercules, CA, USA) antibodies were used, respectively.

Software:

Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
Article Snippet: .. Refractive index (RI) values were analyzed using STEVE software from Nanolive to digitally stain and distinguish nanoplastics from the cytoplasm inside the cells. .. Immunofluorescence staining was performed as previously described.5,24 For the detection of microglia and M1-activated immune cells, Iba1 (1 : 1000, FUJIFILM Wako Chemical, Osaka, Japan) and CD68 (1 : 100, Bio-Rad, Hercules, CA, USA) antibodies were used, respectively.

Article Title: Consequences of platelet-educated cancer cells on the expression of inflammatory and metastatic glycoproteins.
Article Snippet: .. Results were analyzed using EVE Analytics and STEVE software (Nanolive). .. The cell dry mass is calculated using the Analysis Standard module, which allows the dry mass of a cell to be estimated from its refractive index, based on the methods described by Philips et al. and Sandoz et al. [15,16].

Article Title: Cellular immune responses of bovine polymorphonuclear neutrophils to Calicophoron daubneyi
Article Snippet: Image acquisition was set for refractive index (RI; 3D tomography) and blue channel detection, applying timelapse settings (image acquisition over 120 min) using a 3D Cell Explorer-fluo (Nanolive). .. At the end of the experiment, each channel was exported separately using Steve software v.1.6 (Nanolive) and managed with Image J software (Fiji version 1.7, NIH). ..

Article Title: Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net
Article Snippet: The cells were then seeded into collagen-coated 35 mm dishes with polymer coverslip bottoms (ibidi GmbH, Germany) for imaging. .. Holotomographic microscopy (HTM) imaging was performed using a CX-F HTM system (Nanolive SA, Switzerland) connected to the STEVE software (Nanolive SA, Switzerland). ..

Article Title: Multimodal antiproliferative effects of oleanolic acid mitocans: In vitro and in vivo studies.
Article Snippet: Mitochondria-targeting drugs (mitocans) based on organic cations are emerging as powerful and selective cancer therapeutics.. In this study, we have evaluated a novel series of oleanolic acid-derived mitocans, revealing nanomolar-range antiproliferative effects against human solid tumor cells.. Continuous live-cell imaging revealed extensive cytoplasmic vacuolation, while mechanistic studies identified paraptosis as the dominant form of cell death.

Article Title: The correlation of immunotoxicity with size and chemical properties of particulate matter within macrophages.
Article Snippet: In recent years, air pollution has become a major public health concern.. The emission of greenhouse gases aggravates climate change, thereby affecting air quality.. Among these pollutants, particulate matter (PM) can cause respiratory and cardiovascular damage.

Article Title: Mechanistic insights into the anti-aging effects of Crocus sativus in a D-Gal-induced in vitro neural senescence model
Article Snippet: Cells were inspected using a 3D Cell Explorer-fluo microscope (Nanolive) equipped with a dry 60 × /0.8 objective and CMOS camera Sony. .. Acquired quantitative holotomografic images were segmented and analyzed with built-in Eve and Steve software (Nanolive). ..

Article Title: Rab10-associated tubulation as an early marker for biogenesis of the assembly compartment in cytomegalovirus-infected cells
Article Snippet: .. The time-lapse images were saved as 3D stacks using STEVE software (Nanolive SA, Ecublens, Switzerland), which controls the 3D Cell Explorer microscope. ..

Staining:

Article Title: Type-dependent effects of nanoplastics on microglial activation and CXCR2-mediated chemotactic migration.
Article Snippet: .. Refractive index (RI) values were analyzed using STEVE software from Nanolive to digitally stain and distinguish nanoplastics from the cytoplasm inside the cells. .. Immunofluorescence staining was performed as previously described.5,24 For the detection of microglia and M1-activated immune cells, Iba1 (1 : 1000, FUJIFILM Wako Chemical, Osaka, Japan) and CD68 (1 : 100, Bio-Rad, Hercules, CA, USA) antibodies were used, respectively.

Microscopy:

Article Title: Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net
Article Snippet: The cells were then seeded into collagen-coated 35 mm dishes with polymer coverslip bottoms (ibidi GmbH, Germany) for imaging. .. Holotomographic microscopy (HTM) imaging was performed using a CX-F HTM system (Nanolive SA, Switzerland) connected to the STEVE software (Nanolive SA, Switzerland). ..

Article Title: Rab10-associated tubulation as an early marker for biogenesis of the assembly compartment in cytomegalovirus-infected cells
Article Snippet: .. The time-lapse images were saved as 3D stacks using STEVE software (Nanolive SA, Ecublens, Switzerland), which controls the 3D Cell Explorer microscope. ..

Imaging:

Article Title: Label-Free Visualization and Segmentation of Endothelial Cell Mitochondria Using Holotomographic Microscopy and U-Net
Article Snippet: The cells were then seeded into collagen-coated 35 mm dishes with polymer coverslip bottoms (ibidi GmbH, Germany) for imaging. .. Holotomographic microscopy (HTM) imaging was performed using a CX-F HTM system (Nanolive SA, Switzerland) connected to the STEVE software (Nanolive SA, Switzerland). ..

Fluorescence:

Article Title: Multimodal antiproliferative effects of oleanolic acid mitocans: In vitro and in vivo studies.
Article Snippet: Mitochondria-targeting drugs (mitocans) based on organic cations are emerging as powerful and selective cancer therapeutics.. In this study, we have evaluated a novel series of oleanolic acid-derived mitocans, revealing nanomolar-range antiproliferative effects against human solid tumor cells.. Continuous live-cell imaging revealed extensive cytoplasmic vacuolation, while mechanistic studies identified paraptosis as the dominant form of cell death.



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Live <t>3D-holotomographic</t> microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) <t>3D</t> <t>reconstruction</t> and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.
Software Steve, supplied by NanoLive Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/steve+software/steve+software/pmc11865088-81-2-8
Average 90 stars, based on 1 article reviews
software steve - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Live 3D-holotomographic microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) 3D reconstruction and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.

Journal: Frontiers in Immunology

Article Title: Cellular immune responses of bovine polymorphonuclear neutrophils to Calicophoron daubneyi

doi: 10.3389/fimmu.2025.1515419

Figure Lengend Snippet: Live 3D-holotomographic microscopy analyses of bovine neutrophils exposed to Calicophoron daubneyi unveil degranulation and chemotaxis events. (A) Calicophoron daubneyi antigen (100 µg/mL, CdAg) induced degranulation of neutrophils (arrows) as early as 5 min incubation until the end of the experiment. (B) 3D reconstruction and digital staining of holotomographic images confirm shift and accumulation of granules at one side of the neutrophil (zoom, blue arrow). (C) Chemotactic events are recorded between 25 and 54 min of incubation (yellow arrows) with displacement of stimulated neutrophils with CdAg 100 µg/mL. (D) Chemotaxis can be observed after co-culture of neutrophils with Calicophoron daubneyi eggs and analyzed via SEM. Neutrophils are elongated and moving in the direction of the eggs (yellow arrows), while other neutrophils are activated and cast short spr NETs (white arrows) in the vicinity of the eggs. (E) Elongated neutrophils in detail. Rupture is an artefact caused by the SEM analyses.

Article Snippet: For the 3D reconstruction images, the software Steve (Nanolive) was used.

Techniques: Microscopy, Chemotaxis Assay, Incubation, Staining, Co-Culture Assay